Sequence analysis of enzymes with asparaginase activity.

Sequence analysis of enzymes with asparaginase activity.

Asparaginases catalyze the hydrolysis of asparagine to aspartic acid and ammonia. Enzymes with asparaginase activity play an important role both in the metabolism of all living organisms as well as in pharmacology. The main goal of this paper is to attempt a classification of all known enzymes with asparaginase activity, based on their amino acid sequences. Some possible phylogenetic consequenc...

Enzymes for RNA Sequence Analysis

The common slime mold Physarum polycephalum secretes a variety of hydrolytic enzymes when grown in submersed cultures. Initiated by the isolation in 1969 of a rather crude RNase, proposed by Braun and Behrens as a useful analytical endonuclease, an extended search was conducted in our laboratory, which revealed that actually two distinct RNases occur, largely outweighed by a yet unknown acid ph...

Comparative Study of L-asparaginase Activity in Different Bacterial Species

detection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts

in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...

Physical properties of Acinetobacter glutaminase-asparaginase with antitumor activity.

Acinetobaclet glutaminase-asparaginase has been shown to consist of 4 subunits (molecular weight 33,000) by sedimentation equilibrium in 5.5 M guanidine HCl and electrophoresis in sodium dodecyl sulfate on polyacrylamide gels after cross-linking the protein with dimethyl suberimidate. Moving boundary velocity experiments showed that most of the native enzyme sediments as the tetramer (SZO+ = 7....